Programming Neonate Future Health 

Human cytomegalovirus (HCMV) is the most common viral infection transmitted from the pregnant women to the fetus and the leading infectious cause of congenital neurological disease. The objective of the project is to identify maternal antibodies that are the most efficient at preventing the transmission of HCMV and the least likely to cause placental inflammation and fetal injury. The identification of such antibodies will have a considerable impact on the development of new vaccines and therapies against congenital HCMV infection and potentially also against other pathogens transmitted to the fetus such as the Zika virus.

Structural and functional analyses
Structural and functional analyses

Systems serology allows for the broad characterization of IgG response. Glycosylation profiles of antigen-specific IgG are determined by capilary electrophoresis. Unsupervised and supervised machine learning algorithms are used to identify relationships between glycosylation and functional IgG profiles and correlates of clinical outcomes (adapted from Chung et al. Cell 2015)

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Structural and functional analysis
Structural and functional analysis

For analysis of serum antibody repertoire, peripheral blood memory B cell and plasmablasts are sorted and subjected to hightroughput V gene deep sequencing and antigen-specific antibodies are affinity-purified and subjected to proteomic analysis. The proteomic data are interpreted using the antibody sequence database to identify the CDRH3-derived peptides (adapted from Georgiou et al, Nat Biotechnol 2014)

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Structural and functional analyses
Structural and functional analyses

Systems serology allows for the broad characterization of IgG response. Glycosylation profiles of antigen-specific IgG are determined by capilary electrophoresis. Unsupervised and supervised machine learning algorithms are used to identify relationships between glycosylation and functional IgG profiles and correlates of clinical outcomes (adapted from Chung et al. Cell 2015)

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CMV 2019

Birmingham, Alabama, April 7-11 2019

Annual CYMAF steering committee meeting

Pavia, September 23 2019

UPCOMING EVENTS